CONFIRM INFOZ

Straight Up Conversation: LEAP Innovations CEO Phyllis Lockett

Phyllis Lockett is CEO of LEAP Innovations, which runs an 18-month program preparing educators in 140 Chicago schools how to execute personalized learning. Before beginning LEAP, Phyllis was establishing president and CEO of New Schools for Chicago, where she helped raise more than $70 million to contribute to open 80 new sanction schools. I as of late conversed with Phyllis about LEAP and how to prepare teachers in actualizing personalized learning.

Rick Hess: So, what does LEAP do?

Phyllis Lockett: We need to make custom-made learning pathways for each and every understudy. Our work gives locale and schools necessary to change the executives backing to make supportable movements to a personalized learning model. Jump's most significant program, the Pilot Network, is an 18-month program that furnishes educators with direct front arranging, proficient improvement and structure, on-the-ground instructing, and association with personalized learning rehearses and a curated tech item—we accumulate a board of inward and outside specialists to assess edtech devices for adequacy against our customized learning framework, and schools are then coordinated to the item that best meets their requirements. The teacher preparing project incorporates a six-month arranging procedure, and afterward, an entire year of nonstop hands-on help from our group of mentors. We are focused on helping schools actualize the best practices and versatile innovation devices, which help educators tailor the learning knowledge for each understudy.

Rick: What's the enormous thought behind it? What's more, how massive is the association?

Phyllis: LEAP Innovations is established on the conviction that all kids, paying little respect to race, social foundation or financial status, have boundless potential and the privilege to a top-notch instruction. At LEAP, our build for the eventual fate of training is grounded in our framework for personalized learning. Integrating discoveries from neuroscience, learning science, instructional improvement, and the universe of work into a strong, noteworthy vision for schools, the LEAP Learning Framework gives educators robust methodologies to tailor a personalized learning plan to the requirements and chances of their learning surroundings. Our association works in tight organization with the distinct neighbourhood and national establishments, over 2400 educators, and 140 schools crosswise over Chicagoland. All the while, we've affected about 40,000 understudies, 90 percent of whom are offspring of shading, and more than 80 percent of whom are qualified for nothing or discounted value lunch. Our framework has additionally authoritatively been received by the territory of Utah and utilized by schools in 23 states as a major aspect of their work to make learning progressively close to home.

Rick: How would you figure out which schools and teachers take part?

Phyllis: We have expected to fabricate a model that meets schools and networks—just as understudies—where they are. While we work with a various cluster of open, contract and archdiocese schools, we by and extensive search for situations with reliable, comprehensive managerial authority and a culture of coordinated effort and drive for development. Generally speaking, it's essential that each school's needs and objectives line up with the estimations of personalized learning, and that school heads are happy to join forces with their teachers, giving them the opportunity and backing to improve starting from the earliest stage.

Rick: You referenced on-the-ground mentors for these teachers. What do the mentors really do?

Phyllis: After a six-month arranging period, during which educators make and sharpen their study hall plan, our mentors work close by teachers for a full school year as they set their structures to work. They are full-time colleagues, every single previous educator themselves. Mentors visit educators in any event once like clockwork to give nearby help and are accessible as needs are for normal get in contact and direction. They mentor educators through three development cycles, through which they help teachers guide out plans that push toward their enormous picture objectives, assemble estimations that benchmark their advancement, and actualize new practices.

Rick: What sorts of work do teachers do throughout their interest?

Phyllis: For the initial half-year of the Pilot Network, school groups work seriously with our expert advancement group. Our mentors help educators manufacture their structure and set clear plans. Taking interest educators work together intimately with their regulatory groups—and one another—and direct a progression of learning visits to watch propelled schools in real life. As a significant aspect of their plan procedure, teachers make a profile of each understudy in their study hall. This pursues large gatherings with every understudy and frequently begins with "sympathy strolls" to continue the way, indeed, that understudies take to and through school. Teachers at that point plan the various modalities of learning they'll bolster. These incorporate little gathering guidance, kids working together on activities or issues, and students working freely. As opposed to doled out seats and mechanical style columns of work areas, the situations are totally reset frequently to incorporate coordinated effort zones, delicate seating, and understudy portfolio "libraries." Teachers redo their timetables to suit expanded understudy decision and mentorship, setting time for understudy drove conferencing, little gathering guidance, and free tasks. En route, we help educators distinguish ways for understudies to show authority. Parent-teacher meetings are reframed to move toward becoming understudy driven gatherings, where students control discussions about their advancement and where they need more help.

Rick: How have another workforce at the schools responded to the teachers who take part and to the pilot ventures?

Phyllis: There is, as you most likely are aware, a ton of "activity exhaustion" out there. Be that as it may, when teachers see their partners making all the more captivating, personalized homerooms, they unavoidably need to participate. Those underlying study halls become models that the entire school can rally around as educators advance with their own interpretation of personalized learning. This has implied that, during LEAP's initial five years inactivity, the request has developed each year. Of the schools that have taken an interest in our Pilot Network program, about 40 percent have drawn in various groups for different years. Teachers become interested when they see their friends re-stimulated with the experience.

Rick: What's been the response from principals and focal workplaces?

Phyllis: They are excited. They see the positive effect both in their schools' exhibition, and all the more significantly, with the improvement of their students. Our principals state that this synergistic methodology, which carries teachers to the table as co-pioneers, has changed their school societies. As educators and principals perceive the capability of this new methodology, Chicago Public Schools has ventured up to fulfill the need. Personalized learning has shown up unmistakably in the locale's 5-year plan, and it's made another office to help the extension of personalized learning.

Rick: How would you know how powerful the program has been? What sorts of assessments have been directed?

Phyllis: Our initial outcomes are demonstrating twofold digit percentile point gains in proficiency. Schools are announcing higher participation rates and lower suspension rates. Above all, understudies are eager to be in school. Take Perkins Bass Elementary in Englewood. After executing school-wide personalized learning, Perkins Bass has totally changed from a battling Level 3 school—on post-trial supervision for a long time—to a Level 1 school throughout the previous two years. In that time, understudies' math and perusing scores have significantly increased. Before personalized learning, teacher maintenance was underneath state and region levels. Presently it surpasses state and region levels, at 89 percent. Even though class size can be as large as 42 understudies, personalized learning makes an engaged, beneficial, and invigorated learning condition. In our subsequent Pilot Network companion, understudies in our ELA pilot picked up a normal of 2.94 test-score focuses over the control bunch on NWEA, a national evaluation that estimates understudy development. As such, a run of the mill understudy in the Pilot Network picked up 13 other percentile focuses over an ordinary correlation understudy beginning with a similar score—i.e., 50th percentile to the 63rd percentile.

Rick: How much does this expense?

Phyllis: Last year, we charged an independent research firm to take a gander at the expenses of executing personalized learning in schools. It revealed that entire school personalized learning models require unobtrusive venture to begin—start-up expenses ran from $338K to $780K over the six schools and $233 to $1,135 on a for every student premise—and demonstrate maintainable without progressing award financing. It is essential to us—and the examination recommends—this is a model that can withstand and continue moving state and neighbourhood spending plans.

Rick: Where does the financing originate from?

Phyllis: We are blessed to have liberal benefactors that permit school groups to get to our administrations at financed rates. Accordingly, the expense per-school group of doing this work is radically lower than the expense of running the program on our end. Our 18-month program costs us $75,000 per school group, however through altruistic endorsing, we can charge schools just $27,500. This incorporates access to our nonstop, personalized proficient learning administrations, just as year-long utilization of one of our thoroughly screened advantageous edtech items for every single partaking understudy. We've raised more than $30 million to help our work. Our contributors have included nearby Chicago pioneers like The Pritzker Foundation and The Crown Family Philanthropies, just as national philanthropies like the Bill and Melinda Gates Foundation, the Michael and Susan Dell Foundation, New Profit, and the Chan Zucker

Trichinella spiralis

Historical aspect

  In 1835, a man died of tuberculosis in St Bartholomew’s Hospital, London. Dr Paget, a first-year student, carried out the autopsy and observed fine hard white inclusions in the muscles. Similar inclusions had been observed by doctors from time to time in the past, but were attributed to commonplace muscle calcification, which quickly blunted the dissecting scalpel. Dr Paget inspected the lesions with a hand lens and quickly recognised their worm-like structure. The name "Trichina
spiralis" was suggested.

   This name Trichina had already been given to a certain fly, however, and the name was later changed to "Trichinella". The discovery of the parasite was published by the famous biologist and palaeontologist Richard Owen, at that time assistant conservator of the museum of the Royal College of Surgeons. In 1859 Rudolph Virchow carried out transmission experiments in
which infected human muscle was fed to a healthy dog. After only 3 to 4 days adult Trichinella worms were found in the dog’s duodenum and jejunum.

   Life cycle

More than 100 species of mammals are susceptible to the infection. Infections with Trichinella spiralis affect chiefly carnivores and omnivores, although infection of horses has also been described. People become infected with this nematode by eating raw or insufficiently cooked infected meat, often pork or wild boar. The larvae of Trichinella spiralis which are in the meat develop in a few
days into adult worms (2-4 mm) in the wall of the small intestine. There they lay larvae (100 m m). These spread via the bloodstream to various muscles, including the heart, where they undergo encapsulation [Trichinella pseudospiralis does not form a capsule]. The larvae cannot continue to survive in the heart. The larvae are localised within the cells of the muscles, which is unique for a worm.

    After penetrating the muscle cell, a larva excretes a number of signal molecules and
proteins, which convert the cell to what is called a nurse cell. In the cell the behaviour of the worm is rather similar to that of a virus. Many of its proteins are glycosylated and often carry an unusual sugar (tyvelose). These proteins are excreted from a special organ in the larva (the stichosome). Various muscle proteins such as actin and myosin change or disappear, nuclear division is  stimulated and mitochondria are damaged. Local angiogenesis is stimulated by excretion of a blood vessel growth factor and new blood vessels, originating from nearby venules, develop and form a network around the infected cell.

  The metabolism of the nurse cell and the parasite is essentially anaerobic. After 1 to 4 months the adult worms die. The larvae in the muscles sometimes survive for years and can remain viable for a long time even in rotting flesh. Trichinella is unique among worms in that all development stages take place in the same host. There is never a free stage outside the mammalian body.

 Symptoms

Infection may be asymptomatic. In typical cases there is diarrhoea, abdominal pain, vomiting and fever a few days after eating infected meat. After 10 days the fever increases, the patient is very ill and debilitated, there are muscle pains and a typical peri-orbital oedema (differential diagnosis acute trypanosomiasis and nephrosis). This oedema is caused by invasion of the small muscles around the
eye. There may be signs of myocarditis, encephalitis, urticaria and asthma. There is often very significant eosinophilia. The myositis causes an increase in the muscle enzymes (creatine phosphokinase, CK). After a few months the symptoms are reduced or disappear. Mild infections are self-limiting.

 Diagnosis

Not many nematodes are found in muscle tissue. Occasionally a migrating third stage larva of Ancylostoma, Toxocara or Gnathostoma may be found (visceral larva migrans). Dracuncula medinensis may also be found in muscle tissue. Another, less common nematode which may be found here is Haycocknema perplexum (Tasmania).

 Prevention

Meat should be well boiled or roasted thoroughly.  Importance of meat inspection. The diaphragm of a slaughtered animal is inspected (the piece of muscle is flattened between two glass slides and examined using transillumination). This technique (trichinoscopy) is not so good for Trichinella pseudospiralis because it is not surrounded by a capsule and is easily missed.

Pig food (which may include infected rats) should be boiled for 30 minutes.

 
To store pork for 10 days at -25° C is generally impractical in developing countries. In the West meat is sometimes irradiated with high doses of gamma rays, which will kill any larvae. Trichinella spiralis nativa is cold-hardy.

BLOOD AND TISSUE NEMATODES

INTRODUCTION

Filariasis is caused by nematodes (roundworms) that inhabit the lymphatics and subcutaneous tissues. Eight main species infect humans. Three of these are responsible for most of the morbidity due to filariasis: Wuchereria bancrofti and Brugia malayi cause lymphatic filariasis, and Onchocerca volvulus causes onchocerciasis (river blindness). The other five species are Loa loa, Mansonella
perstans, M. streptocerca, M. ozzardi, and Brugia timori. (The last species also cause lymphatic filariasis).

   Among the agents of lymphatic filariasis, Wuchereria bancrofti is encountered in
tropical areas worldwide; Brugia malayi is limited to Asia; and Brugia timori is restricted to some islands of Indonesia. The agent of river blindness, Onchocerca volvulus, occurs mainly in Africa, with additional foci in Latin America and the Middle East. Among the other species, Loa loa and Mansonella streptocerca are found in Africa; Mansonella perstans occurs in both Africa and South America; and Mansonella ozzardi occurs only in the American continent. Another tissue invading parasite is Trichinella spiralis whose larval form is found in the muscular tissue of the host animal. Trichinella spiralis is in fact a complex of three closely related worm species.

   They are morphologically identical, but differ in their host specificity and their biochemical characteristics. T. spiralis spiralis occurs in moderate regions and infects mainly pigs. T. spiralis nativa occurs in the polar regions (polar bear, walrus). These parasites are resistant to freezing which is important for meat storage. T. spiralis nelsoni occurs in Africa and southern Europe with a reservoir in wild carnivores and wild pigs. T. britovi and T. pseudospiralis rarely cause infections. T. pseudospiralis can also infect some birds as well as mammals, unlike the other Trichinella species.

Filarial worms

Wuchereria bancrofti

Different species of the following genera of mosquitoes are vectors of W. bancrofti filariasis depending on geographical distribution. Among them are:

Culex (C. annulirostris, C. bitaeniorhynchus, C. quinquefasciatus, and C. pipiens);
Anopheles (A. arabinensis, A. bancroftii, A. farauti, A. funestus, A. gambiae, A. koliensis, A. melas, A. merus, A. punctulatus and A. wellcomei); Aedes (A. aegypti, A. aquasalis, A. bellator, A. cooki, A. darlingi, A. kochi, A. polynesiensis, A. pseudoscutellaris, A. rotumae, A. scapularis, and A. vigilax); Mansonia (M. pseudotitillans, M. uniformis); Coquillettidia (C. juxtamansonia).

During a blood

meal, an infected mosquito introduces third-stage filarial larvae onto the skin of the human host, where they penetrate into the bite wound. They develop in adults that commonly reside in the lymphatics. The female worms measure 80 to 100 mm in length and 0.24 to 0.30 mm in diameter, while the males measure about 40 mm by .1 mm. Adults produce microfilariae measuring 244 to 296 µm by 7.5 to 10 µm, which are sheathed and have nocturnal periodicity, except the South Pacific
microfilariae which have the absence of marked periodicity.

  The microfilariae migrate into the lymph and blood channels moving actively through lymph and blood. A mosquito ingests the microfilariae during a blood meal. After ingestion, the microfilariae loose their sheaths and some of them work their way through the wall of the proventriculus and cardiac portion of the mosquito's midgut and reach the thoracic muscles. There the microfilariae develop into first-stage larvae and subsequently into third-stage infective larvae. The third-stage infective larvae migrate through the haemocoel to the mosquito's prosbocis and can infect another human when the mosquito takes a blood meal.

Onchocerca volvulus

    During a blood meal, an infected blackfly (genus Simulium) introduces third-stage filarial larvae onto the skin of the human host, where they penetrate into the bite wound. In subcutaneous tissues the larvae develop into adult filariae, which commonly reside in nodules in subcutaneous connective tissues Adults can live in  the nodules for approximately 15 years. Some nodules may contain numerous male and female worms. Females measure 33 to 50 cm in length and 270 to 400 µm in diameter, while males measure 19 to 42 mm by 130 to 210 µm. In the subcutaneous nodules, the female worms are capable of producing microfilariae for approximately 9 years.

   The microfilariae, measuring 220 to 360 µm by 5 to 9 µm and unsheathed, have a life span that may reach 2 years. They are occasionally found in peripheral blood, urine, and sputum but are typically found in the skin and in the lymphatics of connective tissues. A blackfly ingests the microfilariae during a blood meal. After ingestion, the microfilariae migrate from the blackfly's midgut through the haemocoel to the thoracic muscles. There the microfilariae develop into first-stage larvae and subsequently into third-stage infective larvae. The third-stage infective larvae migrate to the blackfly's proboscis and can infect another human when the fly takes a blood meal.

Loa loa

 

  The vector for Loa loa filariasis are flies from two species of the genus Chrysops, C. silacea and C. dimidiata. During a blood meal, an infected fly (genus Chrysops, day-biting flies) introduces third-stage filarial larvae onto the skin of the human host, where they penetrate into the bite wound. The larvae develop into adults that commonly reside in subcutaneous tissue. The female worms measure
40 to 70 mm in length and 0.5 mm in diameter, while the males measure 30 to 34 mm in length and 0.35 to 0.43 mm in diameter.

  Adults produce microfilariae measuring 250 to 300 µm by 6 to 8 µm, which are sheathed and have diurnal periodicity. Microfilariae have been recovered from the spinal fluids, urine, and sputum. During the day they are found in peripheral blood, but during the noncirculation phase, they are found in the lungs. The fly ingests microfilariae during a blood meal. After ingestion, the microfilariae lose their sheaths and migrate from the fly's midgut through the haemocoel to the thoracic muscles of the arthropod. There the microfilariae develop into first-stage larvae and subsequently into third-stage infective larvae. The third-stage infective larvae migrate to the fly's proboscis and can infect another human when the fly takes a blood meal.

Brugia malayi

 

The typical vector for Brugia malayi filariasis are mosquito species from the genera Mansonia and Aedes. During a blood meal, an infected mosquito introduces third-stage filarial larvae onto the skin of the human host, where they penetrate into the bite wound. They develop into adults that commonly reside in the lymphatics.

  The adult worms resemble those of Wuchereria bancrofti but are smaller. Female worms measure 43 to 55 mm in length by 130 to 170 µm in width, and males measure 13 to 23 mm in length by 70 to 80 µm in width. Adult produce microfilariae, measuring 177 to 230 µm in length and 5 to 7 µm in width, which are sheathed and have nocturnal periodicity. The microfilariae migrate into the lymph and enter the blood stream reaching the peripheral blood.

   A mosquito ingests the microfilariae during a blood meal. After ingestion, the microfilariae lose their sheaths and work their way through the wall of the proventriculus and cardiac portion of the midgut to reach the thoracic muscles. There the microfilariae develop into first-stage larvae and subsequently into third-stage larvae. The third-stage larvae migrate through the haemocoel to the mosquito's prosbocis and can infect another human when the mosquito takes a blood meal.

Clinical Features and Pathology

   Lymphatic filariasis most often consists of asymptomatic microfilaremia. Some patients develop lymphatic dysfunction causing lymphedema and elephantiasis (frequently in the lower extremities) and, with Wuchereria bancrofti, hydrocele and scrotal elephantiasis. Episodes of febrile lymphangitis and lymphadenitis may occur. Persons who have newly arrived in disease-endemic areas can develop
afebrile episodes of lymphangitis and lymphadenitis. An additional manifestation of filarial infection, mostly in Asia, is pulmonary tropical eosinophilia syndrome, with nocturnal cough and wheezing, fever, and eosinophilia.

    Onchocerciasis can cause pruritus, dermatitis, onchocercomata (subcutaneous nodules), and lymphadenopathies. The most serious manifestation consists of ocular lesions that
can progress to blindness. Loiasis (Loa loa) is often asymptomatic. Episodic angioedema (Calabar swellings) and sub-conjunctival migration of an adult worm can occur. Infections by Mansonella perstans, which is often asymptomatic, can be associated with angioedema, pruritus, fever, headaches, arthralgias, and neurologic manifestations.

   Mansonella streptocerca can cause skin manifestations including pruritus, papular eruptions and pigmentation changes. Eosinophilia is
often prominent in filarial infections. Mansonella ozzardi can cause symptoms that include arthralgias, headaches, fever, pulmonary symptoms, adenopathy, hepatomegaly, and pruritus.

Treatment and control

Ivermectin is effective in killing the larvae, but does not affect the adult worm. Preventive measures include vector control, treatment of infected individuals and avoidance of black fly.

 Laboratory Diagnosis of filarial worms

Identification of microfilariae by microscopic examination is the most practical diagnostic procedure.

Microscopy

   Examination of blood samples will allow identification of microfilariae of Wuchereria bancrofti, Brugia malayi, Brugia timori, Loa loa, Mansonella perstans, and M. ozzardi. It is important to time the blood collection with the known periodicity of the microfilariae. The blood sample can be a thick smear, stained with Giemsa or haematoxylin and eosin. For increased sensitivity, concentration techniques can be used.

   These include centrifugation of the blood sample lyzed in 2% formalin (Knott's technique), or filtration through a Nucleopore® membrane. Examination of skin snips will identify microfilariae of Onchocerca volvulus and Mansonella streptocerca. Skin snips can be obtained using a corneal-scleral punch, or more simply a scalpel and needle. The sample must be allowed to incubate for 30 minutes to 2 hours in saline or culture medium, and then examined for microfilariae that would have migrated from the tissue to the liquid phase of the specimen.

Preparing Blood Smears for Microscopy Examination

If one uses venous blood, blood smears should be prepared as soon as possible after collection (delay can result in changes in parasite morphology and staining characteristics).

  Life cycle of Human Hookworms

Pathology of Infection.

     The Pathology associated with hookworm infections may be divided roughly into two areas. Firstly the pathology associated with the presence of the adult parasite in the intestine, and secondly the pathology associated with the penetration of, and migration of the larval worms within the skin. The adult hookworms attach themselves to the intestinal wall using their buccal capsules. Their preferred site of infestation is in the upper layer of the small intestine, but in very heavy Adults in intestinal mucosa infections (where many thousands of worms may be present) the parasites may
spread down as far as the lower ileum.

   Once attached to the intestinal wall, the hookworm mouthparts penetrate blood vessels, and the parasites obtain nutrition by sucking blood. A single Necator americanus will take approximately 30 µl of blood daily, while the larger Ancylostoma duodenale will take up to 260 µl. The gross pathology of the disease is very dependent on the intensity of infection. Light infections appear asymptomatic, but in heavy infections, the continuous loss of blood leads to a chronic anaemia, with down to 2gm of haemoglobin per 100ml of blood in extreme cases. Experiments carried out in the 1930's showed that in dogs infected with 500 Ancylostoma caninum a similar species to the human parasite, nearly a pint of blood a day was lost.

  This leads to permenant loss of iron and many blood proteins as well as blood cells. This in turn has consequences for further production of erythrocytes, which have been shown to contain less haemoglobin, as well as being reduced in size and smaller in numbers. This form of anaemia may be directly fatal, but more often, it induces more non-specific
symptoms, the most noticeable being the severe retardation in growth and development, both physical and mental, in infected children, and a general weakness and lassitude, often wrongly interpreted as "laziness".

Diagnosis

Identify characteristic eggs in feacal samples. Note the eggs of N. americanus and A. duodenale are morphologically identical

Epidemiology and Control
 
The factors of epidemiological importance include;
- Poor sanitation through contamination of soil through direct defaecation on the ground.
- Skin exposure to infections e.g. by walking about bare-footed
- Favourable environmental conditions that enhance eggs and larval development.
- Loose, humus soil with reasonable drainage and aeration
- Even distribution of rainfall throughout the year.

Control is by improvement in the standard of sanitation, raising the nutritional status of the population especially in relation to iron content, and mass treatment with suitable worm expeller (vermifuge)

Trichuris trichiura (human whipworm)

The first written record of Trichuris trichiura was made by Morgani, an Italian
scientist, who identified the presence of the parasite in a case of worms residing in
the colon in 1740. Exact Morphological description and figures were first recorded
in 1761 by Roedere, a German physicist. Soon after morphology and visual
representation of the worms, Trichuris trichiura was given taxonomy (during the
18th century).
This is the third most common round worm of humans. It is distributed
worldwide, with infections more frequent in areas with tropical weather and poor sanitation practices, and among children. It is estimated that 800 million people
are infected worldwide. The southern United States is endemic for trichuriasis.

Morphology

Adult worms are usually 3–5 cm long, with females being larger than males as is typical of nematodes. The thin, clear majority of the body (the anterior, whip-like end) is the oesophagus, and it is the end that the worm threads into the mucosa of the colon. The widened, pinkish gray region of the body is the posterior, and it is the end that contains the parasite’s intestines and reproductive organs.Trichuris trichiura has characteristic football-shaped eggs, which are about 50-54µm long
and contain polar plugs (also known as refractile prominences) at each end.

Life cycle and transmission

  Humans can become infected with the parasite due to ingestion of infective eggs by mouth contact with hands or food contaminated with egg-carrying soil. However, there have also been rare reported cases of transmission of Trichuris trichiura by sexual contact. Some major outbreaks have been traced to contaminated vegetables (due to presumed soil contamination).

   Unembryonated eggs (unsegmented) are passed in the faeces of a previous host to the soil. In the soil, these eggs develop into a 2-cell stage (segmented egg) and then into an advanced cleavage stage. Once at this stage, the eggs embryonate and then become infective, a process that occurs in about 15 to 30 days).

   Next, theinfective eggs are ingested by way of soil-contaminated hands or food and hatch inside the small intestine, releasing larvae into the gastrointestinal tract. These larvae burrow into a villus and develop into adults (over 2–3 days). They then migrate into the cecum and ascending colon where they thread their anterior portion (whip-like end) into the tissue mucosa and reside permanently for their year-long life span. About 60 to 70 days after infection, female adults begin to release unembryonated eggs (oviposit) into the cecum at a rate of 3,000 to 20,000 eggs per day, linking the life cycle to the start.

Signs and symptoms

  Light infestations are frequently asymptomatic (have no symptoms). Heavier infestations, especially in small children, can present gastrointestinal problems including abdominal pain and distention, bloody or mucous-filled diarrhoea, and tenesmus (feeling of incomplete defecation, generally accompanied by involuntary straining). While damage may be done to the GI tissue and appendicitis may be brought on (by damage and oedema of the adjacent lumen) if there are large numbers of worms or larvae present, it has been suggested that the embedding of the worms into the ileo-cecal region may also make the host susceptible to bacterial infection.

  Severe infection may also present with rectal prolapse, although this is typically seen only in heavy infections of small children. High numbers of embedded worms in the rectum cause oedema, which causes the rectal prolapse. The prolapsed, inflamed and oedematous rectal tissue may even show visible worms. Growth retardation, weight loss, nutritional deficiencies, and anaemia (due to long-standing blood loss) are also characteristic of infection, and these symptoms are more prevalent and severe in children.

Diagnosis

   A stool ova and parasites examination reveals the presence of typical whipworm eggs. Typically, the Kato-Katz thick-smear technique is used for the identification of the Trichuris trichiura eggs in the stool sample. Although colonoscopy is not typically used for diagnosis, but there have been
reported cases in which colonoscopy has revealed adult worms. Colonoscopy can directly diagnose trichuriasis by identification of the threadlike form of worms with an attenuated, whip-like end. Colonoscopy has been shown to be a useful diagnostic tool, especially in patients infected by only a few male worms and with no eggs presenting in the stool sample.

Epidemiology

     Trichuris trichiura is the third most common nematode (roundworm) of humans. Infection of trichuris trichiura is most frequent in areas with tropical weather and poor sanitation practices. Trichuriasis occurs frequently in areas in which human feces is used as fertilizer or where defecation onto soil takes place.

   Trichuriasis infection prevalence is 50 to 80 percent in some regions of Asia (noted especially in China and Korea) and also occurs in rural areas of the southeastern United States. Infection is most prevalent among children, and in North America, infection occurs frequently in immigrants from tropical or sub-tropical regions. It is estimated that 600-800 million people are infected worldwide with 3.2 billion individuals at risk.

Control and Prevention

   Improved facilities for faeces disposal have decreased the incidence of whipworm. Handwashing before food handling, and avoiding ingestion of soil by thorough washing of food that may have been contaminated with egg-containing soil are other preventive measures. Mass Drug Administration (preventative chemotherapy) has had a positive effect on the disease burden of trichuriasis in East and West Africa, especially among children, who are at highest risk for infection.

  Improvement of Sewage and Sanitation systems, as well as improved facilities for faeces disposal have helped to limit defecation onto soil and contain potentially infectious faeces from bodily contact. A study in a Brazil Urban Centre demonstrated a significant reduction in prevalence and incidence of geohelminth infection, including trichuriasis, following implementation of a city-wide sanitation programme.

  A 33% reduction in prevalence of trichuriasis and a 26% reduction in incidence of trichuriasis was found in the study performed on 890 children ages 7–14 years old within 24 different sentinel areas chosen to represent the varied environmental conditions throughout the city of Salvador, Bahia, Brazil. Control of Soil Fertilizers has helped eliminate the potential for contact with human faecal matter in fertilizer in soil.

The human hookworms

 

introduction 

   The hookworms belong to the Order Strongylida, a large order, and of great interest as it contains many important pathogens of man and domesticated animals. This order is further subdivided into three Superfamilies, the Strongyloidea (the hookworms in man), and two related groups, the Superfamily ‘Trichostrongyloidea’, intestinal nematodes which are of veterinary importance in many domesticated animals (e.g. Haemonchus contortus in cattle and Nippostrongylus brasiliensis in rodents) and members of the Superfamily ‘Metastrongyloidea’ (the lungworms, in domesticated animals).


  In man, there are two species capable of causing intestinal infections, Ancylostoma duodenale native to parts of Southern Europe, North Africa and Northern Asia parts of Western South America, and Necator Americans in Central and Southern Africa, Southern Asia, Australia and the Pacific Islands. These are very important human pathogens. It has been estimated that there are 1200 million cases of hookworm infection in man annually, of which about 100 million of which are symptomatic infections with accompanying anaemia. Also, the larvae of several species of hookworms infecting domesticated animals may penetrate human skin, causing pathology even though they do not develop to the adult parasites in man.

Morphology

    The adult parasites are small cylindrical worms, 0.5-1.5mm long (Ancylostoma duodenale being slightly larger than Necator americanus ). The posterior end of the male worm is equipped with a normal copulatory bursa, used to hold the female nematode in place during mating. The females themselves have a vulva situated near the centre of the body, slightly anterior in Necator and slightly posterior in Ancylostoma.

  The anterior end of the parasites are formed into a buccal  Scanning electron micrograph of the mouth capsule of Ancylostoma duodenale (left), note the presence of four "teeth," two on each side and Necator americanus (right) capsule, absent in members of the other Strongylida superfamilies, by which the different genera and species within the group may be differentiated. For example, members of the genus, Necator have capsules equipped with cutting plates on the ventral margins and within the tablet itself small dorsal teeth. In contrast members of the family, Ancylostoma has pairs of teeth on the ventral margin of the capsule. The number of teeth will vary between different species of Ancylostoma but is usually between one and four pairs.

The eggs are bluntly rounded, thin-shelled, and are almost indistinguishable between the different species, measuring approximately 60 by 40 µm, the eggs of Ancylostoma being slightly larger than those of Necator.

Life cycle

   The life cycles of all the hookworms are very similar. The eggs are passed in the faeces, once exposed to air they mature rapidly if conditions are right, with both moisture and warmth essential for development. When developed, they hatch to liberate a rhabditiform (i.e. having an oesophagus where a thick anterior region is connected via a neckline region with a posterior bulb) L1 larvae after a few days. These larval nematodes feed on bacteria and organic material in the soil, where they live and grow for about two days before undergoing the first moult.

  After about five days more growth they moult again, to produce a much more slender L3 larvae. The L3 larvae have a much shorter oesophagus, is a non-feeding form, and is the infective form of the parasite. Infection takes place by penetration of the skin, for example, when walking with bare feet over contaminated damp soil, followed by entry into the circulatory system.

    Here they are carried to the heart, and then lungs. Once in the lungs, they are too large to pass through the capillary bed there. Instead, they become trapped, and the burrow through the capillary epithelium, entering the air spaces. They then migrate up through bronchi and trachea and are then swallowed. Once swallowed, they pass into the intestine and bury themselves between the intestinal villi. Here they moult to form the L4 larvae, equipped with a buccal capsule allowing adherence to the gut wall. After about thirteen days post-infection they moult for the final time, producing immature adult worms.

 These mature over three to four weeks (i.e. five to six weeks after infection), then mate and commence egg-laying to complete the life cycle. These parasites show a very high fecundity, female Necator americanus  producing up to 10,000 eggs daily, while female Ancylostoma duodenale produces up to 20,000 eggs daily

SOIL TRANSMITTED HELMINTHS

 INTRODUCTION

   Soil-transmitted helminth (STH) infection is highly endemic in tropical and subtropical areas of sub-Saharan Africa, Asia and Latin America, where up to 2 billion people have active infections. STH disease has mainly remained neglected by the global health community because the people most affected are among the most impoverished and because the disease causes chronic ill health with insidious clinical presentations, rather than severe acute illness or high mortality.

  However, it is now recognised that STH infection causes significant morbidity worldwide with 39 million disability-adjusted life years (DALYs) lost each year - more than those lost to malaria (36 million yearly) and approaching those lost to tuberculosis (47 million annually). Hookworm infection alone causes
a loss of 22 million DALYs.

   Ascaris lumbricoides (large roundworm of man) Infection with this roundworm is widespread, with estimates of the annual incidence of infection being higher than 1500 million cases, or around one quarter of the world's population. In addition to the species in man, Ascaris lumbricoides, a morphologically indistinguishable species Ascaris suum is found in the pig. Other related genera include Parascaris in equines and Toxascaris in a variety of domesticated animals.

Morphology

   The adult Ascaris lumbricoides are largely white, or pinkish-white, cylindrical roundworms, slightly narrower at the head. The more slender male's measure between 10 to 30cm long and have a curved tail with two spicules, but no copulatory bursa. The females are very similar, being slightly larger at between 20 to 35cm long, a vulva approximately a third of the length of the body down from
the head, and have a blunt tail. They are both characterised by having a smooth, finely striated, cuticle, and a mouth, which is characteristic of all of the Ascarids (e.g. Toxocara), having three lips each equipped with small papillae. Internally they follow the generalised body plan of all nematodes and have a cylindrical oesophagus opening into a flat ribbon-like the intestine. The eggs consist of a thick transparent inner shell which is covered in a thick, warty, albuminous coat.

Life cycle

  These parasites have a direct life cycle, with no intermediate hosts. The adult parasite lives in the lumen of the small intestine of man, usually only feeding on the semi-digested contents of the gut, although there is some evidence that theycan bite the intestinal mucous membrane and feed on blood and tissue fluids. The female parasite is highly prolific, laying an estimated 2 million eggs daily. In the intestine, these only contain an unembryonated mass of cells, differentiation occurring outside the host.

 This requires a temperature less than 30°C, moisture and oxygen, before the development of the young L1 larvae after approximately 14 days. Eggs containing the L2 larvae take another week to develop, before they are infective to man, and may remain viable in the soil for many years if conditions are optimal. Infection occurs on ingestion of raw food, such as fruit or vegetables, that is contaminated with these infective eggs. The eggs then hatch in the small intestine, to release the L2 rhabditiform larvae (measuring approximately 250 by 15µm in size.

  These do not only grow into the adult forms in the intestine, but must then undergo a migration through the body of their host. These L2 larvae penetrate the intestinal wall, entering the portal blood stream, and then migrate to the liver, then heart, then after between 1 to 7 days, the lungs. Here they moult twice on the way to form the L4 larvae, (measuring approximately 1.5mm Long), then burrow out of the blood vessels, entering the bronchioles. From here they migrate up through the air passages of the lungs, to the trachea. They then enter the throat and are swallowed, finally ending up in the small intestine where they mature and mate, to complete their life cycle

Pathology of Infection.

The majority of infections (~85%) appear to be asymptomatic, in that there is no gross pathology seen. However, the presence of these parasites seems to be associated with the same general failure to thrive in their hosts seen with many of these intestinal nematodes. In terms of more easily identified pathology, this may be divided into three areas; Pathology Associated with the Ingestion and Migration of Larvae Severe symptoms of Ascaris infection may be associated with the migrating
larvae, particularly in the lungs.

   If large numbers of these larvae are migrating through the lungs simultaneously, this may give rise to severe haemorrhagic pneumonia. More commonly, as is the case with most infections, the haemorrhages are smaller in scale, but still may lead to breathing difficulties, pneumonia and/or fever.

  A complication here is that many of the parasites proteins are highly allergenic. Due to this, the presence of the migrating larvae in the lungs is often associated with allergic hypersensitivity reactions such as asthmatic attacks, pulmonary infiltration and urticaria and oedema of the lips. Pathology Associated with Adult Parasites in the Intestine The most common symptoms of infection are due to the adult parasite, and consist of rather generalised digestive disorders, such as a vague abdominal discomfort, nausea, colic (e.t.c.).

  These symptoms are dependent to some extent on the parasite’s burden of the host, which in severe cases may consist of many hundreds or even thousands of parasites, although these are extreme cases. In the case of
these heavy infections, the presence of many of these large parasites may contribute to malnutrition in the host, especially if the hosts (often children) are undernourished.

  A more serious, and potentially fatal, the condition may arise in these more heavy infections, where the mass of worms may block the intestine and need to be surgically removed. This may also occur sometimes on treatment for other. intestinal nematodes such as hookworms, where the curative drug dose for these parasites irritate the ascarids. Pathology due to "Wandering" Adults outside of the Intestine Adult parasites often leave the small intestine to enter other organs, (sometimes in
response to anti-helminthic drugs used to treat other intestinal nematode infections), where they may cause various types of pathology, sometimes with severe consequences.

  For example, adult Ascaris worms may migrate to the bile duct, which may then become blocked, causing jaundice and general interference in fat metabolism. Adult parasites may also migrate to the appendix, or through the intestinal wall, both conditions which may cause fatal peritonitis as they may well carry intestinal bacteria to these sites. They may, alarmingly, sometimes migrate forward through the intestinal tract, to be either vomited up or emerging through the nose. More seriously, if they enter the trachea, they may cause suffocation.

Diagnosis

Definitive diagnosis is by the demonstration of the characteristic eggs in faecal samples or by identifying adult worms passed out spontaneously by the host.

 Epidemiology and Control

Infection occurs through ingestion of parasites’ eggs in the food. The eggs are highly resistant to adverse environmental conditions. This with other factors highlighted below are often associated with the transmission of infection;
- Lack of or inadequate waste disposal facilities
- Improper washing of hands before eating
- Incorrect washing of fruits and vegetables before consumptions
- Unkept rooms and dwelling places that harbour mechanical carriers of parasites, etc.
Provision of the good waste disposal system and good personal hygiene will help to control infections.

GENERAL FEATURES AND LIFE CYCLES OF NEMATODES

INTRODUCTION

Nematode infections in humans include ascariasis, trichuriasis, hookworm, enterobiasis, strongyloidiasis, filariasis, and trichinosis, among others. The phylum Nematoda, also known as the roundworms, is the second-largest phylum in the animal kingdom, encompassing up to 500,000 species. Members of Nematoda are elongated, with bilaterally symmetric bodies that contain an intestinal system and a large body cavity.


   Many roundworm species are free-living in nature. Recent data have demonstrated that approximately 60 species of roundworms parasitize humans. Intestinal roundworm infections constitute the largest group of helminthic diseases in humans. According to a
2005 report by the World Health Organization (WHO), approximately 0.807-1.221 billion humans have ascariasis, 604-795 million have trichuriasis, and 576-740 million have hookworm infections worldwide.

General features

  Nematodes are cylindrical rather than flattened; hence the common name roundworm. The body wall is composed of an outer cuticle that has a noncellular, chemically complex structure, a thin hypodermis, and musculature. The cuticle in some species has longitudinal ridges called alae. The bursa, a flap-like extension of the cuticle on the posterior end of some species of male nematodes, is used to grasp the female during copulation.


   The cellular hypodermis bulges into the body cavity or pseudocolour to form four longitudinal cords; a dorsal, a ventral, and two lateral cords which may be seen on the surface as lateral lines. Nuclei of the hypodermis are located in the region of the cords. The somatic musculature lying beneath the hypodermis is a single layer of smooth muscle cells. When viewed in cross-section, this layer can be seen to be separated into four zones by the hypodermal cords. The musculature is innervated by extensions of muscle cells to nerve trunks running anteriorly and posteriorly from ganglion cells that ring the midportion of the oesophagus.


The space between the muscle layer and viscera is the pseudocolour, which lacks a mesothelium lining. This cavity contains fluid and two to six fixed cells (coelomocytes), which are usually associated with the longitudinal cords. The function of these cells is unknown. The alimentary canal of roundworms is complete, with both mouth and anus. The mouth is surrounded by lips bearing sensory papillae (bristles). The oesophagus, a conspicuous feature of nematodes, is a muscular structure that pumps food into the intestine; it differs in shape in different species.
The intestine is a tubular structure composed of a single layer of columnar cells possessing prominent microvilli on their luminal surface.

The excretory system of some nematodes consists of an excretory gland and a pore located ventrally in the mid-oesophagal region. In other nematodes this structure is drawn into extensions that give rise to the more complex tubular excretory system, which is usually H-shaped, with two anterior limbs and two posterior limbs located in the lateral cords. The gland cells and tubes are thought to serve as
absorptive bodies, collecting wastes from the pseudocolour, and to function in osmoregulation.
Nematodes are usually bisexual. Males are usually smaller than females, have a curved posterior end, and possess (in some species) copulatory structures, such as  Structure of nematodes. (A) Female. (B) Male. Transverse sections through the mid-region of the female worm (C) and through the  oesophagal region (D).

Spicules (usually two), a bursa, or both. The males have one or (in a few cases) two testes, which lie at the free end of a convoluted or recurved tube leading into a seminal vesicle and eventually into the cloaca. The female system is tubular also and usually is made up of reflexed ovaries. Each ovary is continuous, with an oviduct and tubular uterus. The uteri join to form the vagina, which in turn opens to the exterior through the vulva.

A copulation between a female and a male nematode is necessary for fertilization except in the genus Strongyloides, in which parthenogenetic development occurs (i.e., the development of an unfertilized egg into a new individual). Some evidence indicates that sex attractants (pheromones) play a role in heterosexual mating. During copulation, sperm is transferred into the vulva of the female. The sperm enters the ovum, and a fertilization membrane is secreted by the zygote. This membrane gradually thickens to form the chitinous shell.

A second membrane, below the shell, makes the egg impervious to essentially all substances except carbon dioxide and oxygen. In some species, a third proteinaceous membrane is secreted as the egg passes down the uterus by the uterine wall and is deposited outside the shell. Most nematodes that are parasitic in humans lay eggs that, when voided, contain either an uncleaved zygote, a group of blastomeres, or a completely formed larva. Some nematodes, such as the filariae and Trichinella spiralis, produce larvae that are deposited in the host.

Echinococcus spp

 

 

Three species of Echinococcus have been generally accepted as parasites of man

Echinococcus granulosus

   The causative agent of Hydatid disease in man and many other mammals. The dog acts as the definitive host for this species. A number of sub-species of this parasite have also been described, the most universally accepted being E. g. granulosus (thought to be the original species found in Europe, although now more widespread) and E. g. canadensis (the indigenous species of the Arctic region of
North America, for more details see below). In addition there is considerable strain variation within this parasite, with differing preferences for intermediate hosts. For example in Ireland a strain exists whose larvae only infect the horse, man being resistant to infection.

Life cycle

Dogs and other canids are parasitised by the adult tapeworm. When shed by the tapeworm, the gravid proglottids disintegrate in the dogs intestine, and eggs which are passed in the faeces, are highly resistant, being able to survive freezing and drying on the ground for up to a year. Many mammals apart from man may act as intermediate hosts, in particular sheep and horses. The situation is highly complex as at least 9 sub-species have been identified, all with different host specificity;
E.g. granulosus - Adult form in most canids apart from the red fox, hydatids in sheep, pigs, cattle, man and many wild ruminants.
E.g. equinus - Adults in canids, hydatids in horses and other Equidae, but probably not man.
E.g. canadensis - Adults in canids, hydatids in caribou, reindeer and man.
E.g. borealis - Adults in canids, hydatids in many cervids and man.

In addition, in parts of Kenya there is a strain or sub-species that is particularly adapted to transmission between man and domesticated dogs.

  The egg enters the host by ingestion, either from contaminated grass (as is the case in infections of herbivorous ruminants), or in the case of man, by contamination, (for example by the dog licking face after it has been cleaning itself) or other examples of bad hygiene, followed by transfer to the mouth. The egg then hatches in the intestine, penetrates the gut wall, and travels via the lymphatic or blood
system throughout the body, from where they lodge within the body tissues.

   The cysts may develop anywhere within its intermediate hosts body, but as the circulatory blood stream passes from the mesenteric blood vessels to the liver, it is in the liver that the majority of the cysts (in about 65% of cases) are found. Next in frequency of infection are the lungs ( about 20%), brain (1%), peritoneal cavity (8%), kidneys (3%) and bone marrow or other organs. Development of the cysts to produce infective protoscolices takes approximately 1 to 2 years. On the death of the intermediate host, either directly by predation on the part of the dog, or by the scavenging of the dead cadaver, (the protosocialises are also highly resistant, being able to survive in carrion for several weeks), the cyst is ingested along with the offal.

   The cyst wall is then digested, liberating the protoscolices which quickly evaginate, penetrating deeply into the crypts of Lieberkuhn, and developing to adult worms in approximately 7 to 9 weeks. Due to the presence of many protoscolices in each hydatid cyst, dogs may be infected with many E. granulosus (tapeworms).

Morphology

Larvae - These Metacestodes (called 'Hydatids') are large, roughly spherical, fluid filled hollow bladders, containing numerous protoscolices (forming the so-called hydatid sand), brood capsules, and daughter cysts which are identical in form to their parent cyst. The cyst wall itself consists of an outer laminated hyaline wall, supporting the whole cyst. Beneath this there is a nucleated germinal layer, studded with developing brood capsules, which may eventually break off to float freely in the fluid filled cyst.

 The protoscolices are formed within the brood capsules, which may rupture to give the free protoscolices in the hydatid fluid. They vary considerably in size depending on where in the body they form, which may be almost any organ of the body. Those found in the liver (the most common organ affected) may be approximately 20cm in diameter, but those found in the peritoneal cavity may sometimes be very much larger, containing several litres of fluid. For example one case has been reported of a cyst 50cm in diameter, containing 16 litre of fluid.


Adults - The adult parasites in the dog represent one of the smallest of the tapeworms. They measure between 3 and 9mm in length, and usually consist of only 3 proglottids, an immature, a mature, and a gravid proglottid. The scolex is globular in shape, and has a prominent rostellum, armed with a double row of between 30 and 36 hooks. The eggs are very similar to those of the genus Taenia,
and measure between 30 and 40µm in diameter

Larvae - In domesticated animals clinical signs appear to be uncommon, whilst inman they will vary in their seriousness depending on where in the body the hydatid develops, and how large it grows. Sometimes, the infection is asymptomatic, the only evidence of infection being the presence of calcified cysts on autopsy after death due to an unrelated cause. The major pathology is due to the
size of the cyst, giving rise to pressure related injury. A complication may arise if the cyst is ruptured, possibly due to blows to the body, muscular strain, or during operations. In this case the contents of the hydatid is released into the body's circulatory system, and the liberated protoscolices may give rise to numerous secondary cysts throughout the body. In addition the hydatid cyst fluid is highly allergenic and cyst rupture may result in anaphylactic shock and rapid death.

Adults - The adult tapeworm is usually non-pathogenic to its canine hosts,
although sometimes in very heavy infections there may be some inflammation of
the intestinal wall.

Pathology of Infection of Taenia

 

 

T. saginata

Larvae - Unlike T. solium, T. saginata does not utilise man as an intermediate host, and therefore pathology due to the larval form is not a feature in human disease. In cattle, the cysticercus, referred to as Cysticercus bovis (named before the parasite life cycle had been determined, and the connection between the two forms had been established) is completely asymptomatic.

Adults - The pathology of infection with adult T. saginata is highly variable. Often infections are completely asymptomatic, but in other cases, some degree of pathology may be seen, most seriously intestinal blockage. In some cases, vitamin deficiency may be the result of excessive absorption of nutrients by the parasite, although this aspect of tapeworm pathology is more a feature of infection with the fish tapeworm D. latum. Also infection may be accompanied by a broad
range of non-specific symptoms, including more commonly, (if seen at all), abdominal pain, digestive disturbances, excessive appetite or loss of appetite, weakness and weight loss.

T. solium

Larvae - Infection with the larval form of T. solium Cysticercus cellulose, (called "Cysticercosis") may have severe consequences, the annual world-wide mortality due to cysticercosis having been estimated at approximately 50 000 cases. In man, the cysticerci mainly develop in the subcutaneous tissues, but infections in both the Central Nervous System (C.N.S.) and ocular tissues are also very common. Infection of the C.N.S. may cause severe pain, paralysis, optical and/or psychic disturbances and epileptic convulsions, mainly due to mechanical pressure as the larvae develop. Later there may be loss of consciousness and even death. Infections involving the eye may give rise to discomfort and can cause detachment of the retina.

Adults - Usually only a single adult specimen is present, which may cause a slight degree of mucosal inflammation. The actual effects on the host may vary considerably, often there are few symptoms, but in some cases, a variety of nonspecific symptoms such as constipation, epigastric pain and diarrhoea, are present.

Exceptionally rarely there may be perforation of the intestinal wall, with subsequent peritonitis. However, more seriously, as detailed above, the presence of adult worms carries the risk of autoinfection due to reverse-peristalsis resulting in cysticercosis, it is estimated that approximately 25% of cases of Cysticercus cellulose infections in the man being acquired by this route.

Diagnosis of Infection by Taenia spp

- Demonstration of scolex and proglottids in the faeces. However, scolex are rarely excreted in faeces
- The eggs of T. saginata and T. solium are similar. However, most laboratory diagnosis is through the observation of Taenia spp eggs in a faecal sample.
- Examination of the gravid uterus shows 15-25 lateral branches in T. saginata and 7-13 lateral branches (counted from the main stem) in T. Solium when short chains of 5-8 proglottids passed out in faeces are pressed through glass slides.
- The scolex of T. saginata is easily distinguished from that of T. solium in that it has only 4 suckers but no hooks.
- Radiological examination of the intestinal tract may reveal tapeworm infection.

Epidemiology and control

The prevalence of Taenia infection is on the increase due to the following factors;
1. Intensification of animal production
2. Development of meat industries in several developing countries
3. Consumption of undercooked beef and pork by a tourist visiting highly endemic areas
4. Consumption of semi-cooked meat in manufactured food products like hamburgers, etc.
5. Accelerating urbanisation with decreased efficiency of sewage systems
6. Sewage farming Given the above listed epidemiological factors that favour transmission,

 the following measures can be taken to reduce prevalence;
 
1. Proper meat inspection services before usage in meat industries. Diseased
meat should be condemned and destroyed
2. Lightly infected beef with cysticerci can be rendered safe for consumption
by freezing at -10oC for at least 10 days
3. Cooking of meat well before eating
4. High standards of sanitation will reduce transmission
5. Immunization against bovine cysticercosis.

Other Taenia cestodes

Infection by Adult tapeworms

Taenia taeniformis - This parasite has a cosmopolitan distribution, the adult parasites are normally found in cats and related carnivores, but it has been reported from an Argentinean child. The adult tapeworms are about 60cm long and are unusual in that they lack a neck. The scolex is large and equipped with two rows of hooks, while the posterior gravid proglottids have a characteristic bell shape. The larvae, which is found in wild rodents, is a strobilaceous, development of a
cysticercus where the scolex has evaginated but is still attached to the bladder of the cysticercus by a short segmented strobila.

Taenia Bremner (Syn. T. confusa) - reported from man in Africa, Japan and the United States of America. This parasite may be a synonym of T. saginata. Taenia africanus - published a few times in East Africa. This tapeworm has broad segments and an unarmed scolex with a small apical sucker.

Infection by Larvae (Metacestode Infections)

Taenia multiceps - The adult tapeworms of this species are found in dogs and related canids. The larva is a fluid containing cyst 5cm or more in diameter, containing several hundred protoscolices, and is called a coenurus. It is normally found in the brain or spinal cord of sheep and goats where it is an important pathogen. In these animals, it causes a condition known as 'gid' or 'staggers' as the
coenurus develops along with associated destruction of nervous tissue. The larval form may rarely infect man, where it causes a condition called coenurus cerebralis, on accidental ingestion of tapeworm eggs from the faeces of dogs.

Taenia serialises - A similar parasite to T. multiceps, the coenurus larvae, measuring 4cm in diameter or larger, is usually found in the subcutaneous and intramuscular tissues of lagomorphs. The adult tapeworms are found in dogs and foxes with a cosmopolitan distribution. They measure about 70 cm in length and have a scolex with two rows of about 30 hooks. The larvae have been reported very rarely in man.

Taenia glomerulus - The larvae commonly infect rodents, but the coenurus larvae have also been reported as rarely affecting man in Africa

TAPEWORMS OF MAN AND OTHER HUMAN’S CESTODES

INTRODUCTION

  Two species from the genus Taenia are common parasites of man, these being Taenia solium (the Pork tapeworm) and Taenia saginata (the Beef tapeworm). Taenia saginata has a cosmopolitan distribution, with estimates of approximately 50 million cases of infection world-wide annually. As with T. saginata and T solium this parasite has a cosmopolitan distribution, with estimates of
approximately 50 million cases of infection world-wide annually.

  However the incidence of infection may vary considerably, and may be influenced by a number of factors such as religious inhibitions on eating pork, as in many Islamic countries, or in other countries by high degrees of sanitation, limiting exposure of the intermediate hosts to human faeces.

   This parasite has pigs as the main intermediate host, but man may also act as an intermediate host for this parasite as well as being infected with the adult tapeworms. This aspect of the parasites lifecycle has important implications for the pathology associated with infection with this parasite.

Echinococcus granulosus is one of the three species of Echinococcus that is generally accepted as parasites of man. It is the causative agent of Hydatid disease in man and many other mammals. It occurs in Europe and Artic region of North America.

Taenia spp

 Life cycle of Taenia spp

   This parasite has cattle or related animals as its main intermediate hosts, although other animals such as camels, llamas and some antelopes may also occasionally be infected. The larval form in these animals is a cysticercus in the muscles and heart. These are infected by ingestion of the eggs of the tapeworm, shed from the faeces of the carnivorous definitive host, in this case man. Once ingested the eggs hatch to release the hexacanth larvae, which migrate through the intestinal wall to reach the blood or lymphatic systems, from where it is carried to the tissues, particularly the heart and other muscles to develop into the cysticercus. Man is infected by ingestion of undercooked or raw meat, the bladder wall of the cysticercus being digested in the intestine to release the scolex of the parasite. This attaches to the intestinal wall and grows into the mature adult tapeworm.

Morphology

Taenia saginata

Larvae - These cysticerci are approximately 7.5-10mm wide by 4-6mm in length.
Adults - The adult tapeworms have an average length of about 5 meters, consisting of approximately 1000 proglottids, but may grow up to 17 metres in length occasionally, and are therefore longer than the adult forms of Taenia solium. The mature proglottids have approximately double the number of testes that T. saginata has and are larger. The gravid proglottids are also larger, measuring approximately 20mm long by 6mm wide with a uterus with more lateral branches than T. solium.

These gravid proglottids when detached from the strobila may be very active, not only crawling away from the faeces when passed, but often actively emerging from the anus to deposit eggs from the ruptured uterus around the perianal region. The scolex in this tapeworm may also be differentiated
from T. solium as it is slightly larger, at approximately 2mm in diameter and is unarmed, without any hooks, although the 4 acetabular suckers are still present.

Taenia solium

Larvae - These small cysticerci (refered to as Cysticercus cellulosae) are approximately 6- 18mm wide by 4 - 6mm in length when found in the muscles or subcutaneous tissues (the normal sites for the larva of this parasite). The cysticerci may however be found in other tissues such as those of the central nervous system where they may grow much larger, up to several centimetres in diameter.

Adults - The adult tapeworms have an average length of about 3 meters, but may grow up to 8 metres in length occasionally, and follow the typical morphology of cestode tapeworms. The strobila consists of between 800 and 1000 proglottids. The mature proglottids having trilobed ovaries with a small central lobe in addition to the two lateral lobes and only approximately half the number of testes that T. saginata has. The gravid proglottids, measuring approximately 12mm long by 6mm wide, have a uterus with between 8 to 12 lateral branches, less than T. saginata. The scolex in this tapeworm may also be differentiated from T. saginata as it is equipped with a low rostellum with a double crown of approximately 30 hooks.

H. diminuta - The Rat Tapeworm

   This tapeworm is much longer than H. nana, growing up to 60cm or more in length. This is primarily a parasite of the rat, humans only being infected by accidental ingestion of the insect intermediate host. This species is of more importance as a research model for the study of the biochemistry, physiology, chemotherapy and immunology of tapeworm infections. In addition there are a number of species found in animals, including;

H. carioca - A common non-pathogenic parasite of fowl in the USA.
H. microstoma - A parasite of rodents.
H. lanceolata - A pathogenic parasite of ducks, geese and other anseriform
domestic fowl.
H. coronula- A parasite of anseriform domestic fowl.
H. cantaniana - A parasite of chickens and other galliform domestic fowl.

Morphology

   Apart from their relative sizes, these two parasites of man are very similar, H. nana being up to 4cm in size, the strobila consisting of up to 200 proglottids, whilst H. diminuta grows up to 60cm or more in length and the strobila consists of up to 1000 proglottids. These proglottids are trapezoidal in shape, and are approximately four times as wide as they are long. Each proglottid contains three
round testes, a bi-lobed ovary, a compact vitelline gland and a large uterus opening to a lateral genital pore (as does the cirrus).

   The scolex in both parasites have four suckers and a retractile rostellum which in H. nana is equipped with 20 - 30 hooks (the rostellum is unarmed in H. diminuta). Finally the eggs of the two species both have the characteristic thickened walls of all cestode eggs, but may
easily be differentiated. Those of the yellowish brown H. diminuta eggs are much rounder than colourless H. nana eggs and are larger with 60 - 80µm in diameter. In H. nana the eggs are oval in shape, measuring ~ 40 by 50µm and contains an oncosphere equipped with 3 pairs of embryonic hooks (i.e. a "hexacanth" larvae) and long wavy filaments (absent in H. diminuta ) which lie in the space between the larvae and the egg shell wall. The two species infecting man have rather different lifecycles which will be considered separately here.

H. nana

    This parasite has rats and mice as well as man as the definitive host, and differs from H. diminuta and almost all other tapeworm in that an intermediate host is not required, although fleas and beetles may be used. The embryonated eggs are passed in the faeces where they contaminate soil. If the eggs are ingested by the definitive host the oncosphere is activated and breaks out of the egg and penetrates the gut villus. Here it develops as a cysticoid larvae in about 4 days before rupturing into the gut lumen. Once ruptured, the scolex attaches to the gut mucosa and the parasite develops into the adult tapeworm after about 15 to 20 days. If the insect intermediate hosts are utilised the lifecycle is similar to that of H. diminuta below. In heavy infections eggs liberated by adult worms in the intestine may hatch here rather than passing out of the body, to give autoinfection

H. diminuta

   This parasite as in most tapeworms does require an intermediate host. Embryonated eggs pass out of the body of the definitive host in the faeces and are ingested by the insect intermediate hosts. Many insects may act as intermediate hosts for this parasite, the most common being fleas and beetles such as the flour beetle. When ingested by the intermediate host the oncosphere larvae become activated, break out of the egg shell and penetrate into the insect’s body cavity where they develop into a cysticercoid larvae. For completion of the lifecycle, the infected intermediate host must be eaten by the definitive host. On ingestion, the cysticercoid larva becomes activated, the scolex becomes attached to the gut mucosal wall, and the parasite develops into the adult tapeworm.

An interesting feature of Hymenolepis tapeworms is that they undergo a diurnal migration within the gut, which is associated with the feeding patterns of the host. From about 4pm to 4am few parasites are seen in the lower part of the small intestine, whilst from about 4am to 4pm many parasites are seen in the upper part of the small intestine. This was first observed in H. diminuta and subsequently in other species, and is indicative of a nocturnal feeding pattern by the parasite.

Pathology of Infection.

 
  These parasites are not very pathogenic, usually with asymptomatic infections. In man infected with H. nana there may be a slight irritation of the gut mucosa and slight abdominal pain, and with very heavy infections (>2000 worms) there may also be some diarrhoea. In the bird species there may be enteritis and intestinal obstruction with some species.

TAPEWORMS AND EXAMPLES

INTRODUCTION

    Cestodes or tapeworms are the most specialised of the Platyhelminthe parasites. All cestodes have at least one, and sometimes more than one, secondary or intermediate host as well as their primary host. While the intermediate hosts are often invertebrates of some sort, the primary host is normally a vertebrate. However, in some cases both hosts are vertebrates, as in the common Beef  Tapeworm (Taenia saginatus), and in a few species there may be only a single host. A number of tapeworms include mankind in their life cycles but infection is not normally a serious health problem and can be cured. There are more than 1,000 species of tapeworms known to science, and nearly every species of
vertebrate is liable to infection from at least one species of tapeworm.

Diphyllobothrium latum (the Broad Fish Tapeworm)

Diphyllobothrium latum is the fish tapeworm of man. It has a fairly c

  osmopolitan distribution, but is particularly common in the Baltic region, Russia and the Great Lakes region of the U.S.A.


Morphology of the adult Tapeworm

   The adult parasites are typically between 2 and 12 m in length by up to 2 cm in width, but may grow even longer in some cases. The anterior organ of attachment is a bothria, a pair of shallow, elongated muscular grooves, typical of tapeworms of the order Pseudophyllidea. The body is divided into proglottids, as is the case of all pseudophyllidean tapeworms. These proglottids are broader than they are long, except at the terminal end, where they are approximately square in shape. Internally the proglottids are typical of pseudophyllidean tapeworms, with numerous testes and vitellaria arranged on the lateral margins of the proglottid, with a central bilobed ovary.

  An important difference between this parasite and the other tapeworms of man is that the uterus open to the exterior (cyclophyllidean
tapeworms have closed uteruses). Eggs are therefore actively deposited by the parasite, in contrast to the disintegration of the proglottids seen in the other human tapeworms.

Life cycle and Transmission
 
Diphylobothrium latum has a typical Pseudophyllidean tapeworm lifecycle. In addition to the adult parasites in the definitive host, (i.e. man), there are two intermediate hosts containing larval stages. Eggs are passed from man in the faeces and hatch in water to release a small motile embryonic parasite, the Coracidium. This is internally similar to the hexacanth larvae of the Cyclophyllidean tapeworms, being equipped with 6 hooks, but this hexacanth larva is covered in a ciliated embryophore. The coracidium is a free swimming stage, but cannot survive long. For further development, it must be ingested by the first intermediate host, a copepod.

  On ingestion the embryonic larvae penetrates the arthropods gut wall, entering the haemocoel to develop into the first larval stage, the procercoid, measuring 50µm in length. This larva, (as well as the next larval stage, the plerocercoid described above) is very different from the cyclophyllidean parasite larvae in that they have elongated and solid bodies. In addition, the procercoid bears the embryonic hooklets on a posterior bulb like rounded growth, the cercomer. To continue the lifecycle, the copepod must be ingested by the next intermediate host, a fish. The procercoid penetrates the gut wall of the fish, and develops into the next larval stage, the plerocercoid (sparganum), measuring 4 - 5 mm in length, in the viscera or musculature of the fish.


  These plerocercoids have again elongated solid bodied parasites, but differ from the procercoids in the absence of the cercomer and hooklets, and at the anterior end having a developed attachment organ, the bothridium, similar to the adult parasite. A number of different species of fish may act as intermediate hosts for the plerocercoids of D. latum, but the highest densities of plerocercoids are found in carnivorous fish such as the pike. These high parasite loads are because, in addition to infection by ingestion of the copepod plus procercoid, if another infected fish is eaten the plerocercoids within the body tissues of this predated fish are released in the intestine of the carnivorous fish.

   These then migrate through the intestinal wall, to invade the new host, which is then acting as a Paratenic host for these secondary plerocercoids. The plerocercoids are, in addition, very long lived, and may achieve very high parasite densities. Man is infected by ingestion of raw or undercooked fish, the plerocercoids emerging in the intestine to grow into the adult parasite. In addition to man a number of other fish eating mammals may also be infected, including cats, dogs, pigs, bears. Therefore D. latum in addition to being a parasite of man, also causes zoonotic infection. In man multiple infections may occur, sometimes of very high numbers (up to 143 worms have been reported from a single individual). In these cases the parasites length is considerably reduced.

Pathology of Infection

  Infection, as is often the case with adult tapeworms, presents a variable range of pathology, but again is not commonly the cause of serious disease in man. Symptoms, when they occur, include a variety of non-specific abdominal signs, including abdominal pain and loss of weight, and are often very similar to the symptoms displayed during infection with adult Taenia. However, D. latum differs
from Taenia in absorbing much more vitamin B12, (between ten and fifty times more) than other tapeworms. Infection may therefore result in a macrocytic hypochromic anaemia in some cases, vitamin B12 having an important role in formation of blood cells. This feature of the disease is much more common in the Baltic region, particularly in Finland. This tapeworm derived anaemia may be due to host derived genetic factors. It is also more commonly seen when the tapeworm
is situated higher in the intestine.

Epidemiology and Control
Infection occurs by consuming raw or undercooked fish harbouring sparganum. Therefore to avoid infection in man, fish should be properly cooked, killing the infective plerocercoids.

Dipylidium caninum - (the Dog Tapeworm)
This Tapeworm is primarily a parasite of the Dog and the Cat. However man, and in particular children, may also be infected.

Lifecycle

Similar to Taenia saginata, the proglottids of this tapeworm are actively motile, and are able to crawl out of the anus of the definitive host as well as being passed in the faeces. The eggs of this species of tapeworm are contained in egg-capsules, each containing up to twenty eggs. These eggs are ingested by the parasite’s intermediate host, in this case an invertebrate arthropod such as fleas (only the
larval flea can be infected) or the dog louse Trichodectes canis.

   The onchosphere larvae is released in the arthropods gut and penetrates through the gut wall, developing into a cysticercoid, similar to the hymenolepid larval tapeworms. Infection of the Definitive host, whether dog, cat or man, occurs on ingestion of the larval parasite, either when the intermediate host is ingested, or ingestion of the crushed bodies of these hosts. For example, if the dog licks the face of the child just after it has bitten a flea or louse. On ingestion the cysticercoid larvae develops into the adult parasite in the small intestine in about twenty days

Morphology

Larvae - The larvae are roughly pear-shaped, and follow the typical cysticercoid body pattern.
Adults - These are relatively short tapeworms, measuring between 15 and 17cm in
length and consisting of up to 170 proglottids. These are elongated in form, the gravid proglottids, measuring approximately 12 x 3m and packed full of eggcapsules, having the appearance of grains of rice. The scolex by which the parasite attaches to the wall of the small intestine has four large acetabulate suckers, a retractile rostellum and six rows of 30 to 150 rose-thorn shaped hooks. The eggs which are typical Cyclophyllidean tapeworm eggs, are round in shape and measure
up to 60µm, and are held within egg-capsules.

Pathology of Infection.

  The infection appears to be asymptomatic and generally non-pathogenic, although there may be some degree of mild pruritis, or itching, around the perineum due to the presence of emerging proglottids.
Tapeworms of the Genus Hymenolepis There are a number of species in this genus, two of which are common parasites of man.

H. nana - The Dwarf Tapeworm

 
   This tapeworm is relatively small, growing up to 4cm in length, the size of the parasite being inversely proportional to the number of worms present in the infection. Infections, which are more commonly seen in children in warmer climates, are characterised by the presence of numerous parasites (both cysticercoid larvae and adults) in the small intestine. Infection is by ingestion of soil contaminated with faeces containing eggs and may give rise to abdominal
discomfort.

Larval Metacercaria

The Larval Cestodes

1) The Cestodarians
 
   The custodian's larvae or lycophore are free swimming, being covered in cilia. They have a set of ten hooks at the extreme anterior of the body, thus differing from the larval cestodes, which are equipped with 3 pairs of hooks. Anteriorly they are armed with penetration glands. The bodily form of these larvae bears a marked resemblance to the larvae of the trematodes, such as the miracidium in the digenean, and the larval monogenean, the oncomiracidium.


The Eucestodes (Tapeworms)
 
The eggs of Pseudophyllidean and Cyclophyllidean cestodes differ considerably. The egg of the pseudophyllidean tapeworm closely resembles that of the trematodes, having a thin shell wall, and an operculum, which on hatching opens to resales the free-swimming larvae. This illustrates the close relationship between the two major groups of platyhelminth parasites. In contrast, the egg of the cyclophyllideans tapeworms is very different, having a very thick, resistant eggshell, with no operculum.

   The larvae emerging from these eggs also differ. The pseudophyllidean egg hatches to release free swimming larvae called a miracidium. This has an outer layer of ciliated epidermal cells with which it swims through the water before being ingested by the parasites first intermediate host. This is often a copepod. Inside the copepod the ciliated epidermis is shed, to release a larva that initially resembles that of the newly hatched cyclophyllideans. This has 6 hooks, arranged in pairs, and is a common feature throughout the cestodes. Based on the presence of these hooks, present in both the cestodes and custodians, many authors believe that the cestodes originally evolved from an ancestor common to the extant monogeneans.


Coracidium larva
 
The larval cyclophyllidean, as with the pseudophyllidean, is equipped with 3 pairs of hooks. Both groups use these hooks to penetrate the gut wall of its intermediate host after being ingested, before developing into the other larval forms described below in more detail.


Metacestodes
 
Several different larval forms of cestodes (metacestodes) are seen, these include the following


   A larval form of Pseudophyllidean cestodes, (e.g. D. Tatum, Ligula intestinalis). Here two types of the progeroid are shown. Firstly an immature progeroid, and secondly a mature infective progeroid. In the lifecycle of these parasites, there are two intermediate hosts. The procercoid being found in the first of these (usually a small crustacean, e.g. Cyclops).In appearance, these larvae have solid bodies with the remains of the embryonic hooks from the onchosphere larvae at the posterior of the parasite.


   A larval form of Pseudophyllidean and other Cestodes, (e.g. D. Tatum, Ligula intestinalis). In
the lifecycle of these parasites there are two intermediate hosts (see the cestode life cycle page). The plerocercoid being found in the second of these (usually a fish or amphibian).
In appearance, these are elongated larvae with solid bodies which are much larger than the
preceding progeroid larvae.

    In these stages the embryonic hooks are absent. The plerocercoids
of some Pseudophyllideans already show the start of the development of the sexual organs
(e.g. Schistocephalus solidus, Ligula intestinalis), while those of Schistocephalus solidus are also already divided into proglottids).

A larval form of Cyclophyllidean Cestodes, (e.g. Hymenolepis sp.). This larval form is usually found in species where the intermediate host is an invertebrate, usually an insect.

A larval form of Cyclophyllidean Cestodes, (e.g. Taenia solium)
A larval form of Cyclophyllidean Cestodes, (e.g. Mesocestoides sp. ).
A larval form of Cyclophyllidean Cestodes, (e.g. Taenia taeniaeformis ).
A larval form of Cyclophyllidean Cestodes, (e.g. Taenia multiceps ).
A larval form of Cyclophyllidean Cestodes, (e.g. Echinococcus granulosus). - Hydatid cyst